Plasmid_Backbone
bkd gibson

Part:BBa_K4074045

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)


pSB_BKD_GIBSON

Contents: bkd operon (gibson)
What is it?: A basic part - plasmid backbone
What does it do?: It codes for bkd operon homology arms, which allow for integration into the B. subtilis chromosome at the bkd operon.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1187
    Illegal suffix found at 3503
    Illegal EcoRI site found at 2344
    Illegal XbaI site found at 2317
    Illegal PstI site found at 2305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 2344
    Illegal SpeI site found at 3504
    Illegal PstI site found at 2305
    Illegal PstI site found at 3518
    Illegal NotI site found at 1193
    Illegal NotI site found at 3492
    Illegal NotI site found at 3511
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 2344
    Illegal BamHI site found at 2323
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1187
    Illegal suffix found at 3504
    Illegal EcoRI site found at 2344
    Illegal XbaI site found at 2317
    Illegal PstI site found at 2305
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1187
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal XbaI site found at 1202
    Illegal XbaI site found at 2317
    Illegal SpeI site found at 3504
    Illegal PstI site found at 2305
    Illegal PstI site found at 3518
    Illegal NgoMIV site found at 3463
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None